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Bioactive Surfaces

Deep-Tip AFM

DeepTip TM probes are functionalized AFM probes produced by the company Bioactive Surfaces S.L. specially adapted for applications in affinity atomic force microscopy (A-AFM) and single molecule force spectroscopy (SMFS) experiments.

DeepTip TM probes present a surface layer with a high density of reactive amine groups. This functionalized layer is highly stable both mechanically and chemically. Their high mechanical stability allows the repetitive contact of the DeepTip TM with the substrate without detectable degradation. Additionally, their high chemical stability allows storage for months under conventional conditions and makes these probes compatible with a wide range of crosslinking chemistries. The variety of crosslinking chemistries, in turn, implies that DeepTip TM can be decorated with essentially any sensor molecule of interest.

DeepTip TM are produced with different base materials and geometries. Among the most common DeepTip TM probes are found the SiN and the SiO series, produced using silicon nitride and silicon oxide respectively as base materials. Typical geometries comprise rectangular and triangular cantilevers with different dimensions. The combination of base material and geometrical features allows producing DeepTip TM with a wide variety of elastic constants and resonance frequencies. In addition, DeepTip TM can be found with different geometries of the tip, including tipless probes.

The possibilities of the DeepTip TM probes are illustrated in various scientific manuscripts. Among the results that can be highlighted, it was shown that it is possible to measure the low intensity interaction between a protein and a lipid with single molecule resolution.

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[Type: Product] [Status: Ready]
Deep-Tip AFM
Bioactive Surfaces

Thermally Activated Biofunctionalization (TAB)

This technology allows the efficient functionalization of metallic surfaces with amino reactive groups for the covalent binding of biological molecules (peptides, proteins and enzymes, particles and others).
This technology allows the functionalization of different materials, including those of intricate geometries. We have demonstrated TAB’s capability to increase the adhesive capacity of mesenchymal stem cells. This results in higher proliferation rates and enhanced differentiation into bone-forming cells compared to other functionalizing techniques.